Glassy State and Cryopreservation of Mint Shoot Tips
نویسندگان
چکیده
منابع مشابه
Glassy state and cryopreservation of mint shoot tips.
Vitrification refers to the physical process by which a liquid supercools to very low temperatures and finally solidifies into a metastable glass, without undergoing crystallization at a practical cooling rate. Thus, vitrification is an effective freeze-avoidance mechanism and living tissue cryopreservation is, in most cases, relying on it. As a glass is exceedingly viscous and stops all chemic...
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The aim of the present study was to investigate the importance of the preculture conditions on the survival of apple (Malus domestica Borkh., cv. Romus4 and rootstock M106) shoot tips cryopreserved using a combined droplet vitrification procedure. Preculture was critical for the survival of apple shoot tips after cryopreservation. Among the different tested sugars and sugar alcohols (glucose, s...
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Many plant species can be cryopreserved by treating shoot tips with complex cryoprotectant solutions before rapidly cooling them to liquid nitrogen temperatures. Plant vitrification solution 2 (PVS2), a commonly selected cryoprotectant, can be lethal with extended exposure times. To determine potentially toxic combinations, we have exposed mint shoot tips to one-, two-, three-, and four-compone...
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Encapsulation-dehydration and PVS2-vitrification cryopreservation protocols were evaluated for the long-term conservation of a diverse group of Rubus germplasm. Cold acclimation for a 4-week period prior to cryopreservation was necessary for regrowth of shoot apices from blackberry and raspberry genotypes. For the encapsulation-dehydration protocol, encapsulated apices were pretreated in 0.75 M...
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ژورنال
عنوان ژورنال: Biotechnology Progress
سال: 2013
ISSN: 8756-7938
DOI: 10.1002/btpr.1711